Luisierella is characterized by its small size, blackish coloration, and rounded to obtuse leaves that are tubulose when dry but have margins plane or incurved when wet. Its upper leaf cells are strongly rounded and mammillose-bulging on the ventral surface but plane and smooth on the dorsal surface. The basal leaf cells are thin-walled, abruptly inflated and continued upwards along the margins in a v-shape pattern very similar to those of Tortella. The costa has a single stereid band, with ventral superficial epidermal cells well-developed. Zander (1993) described the genus as gynodioicous, i.e., with both monoicous and gynoicous plants. Individual plants can be synoicous, perigoniate, or have a mixture of synoicous and perigoniate inflorescences. Its capsules are cylindrical and the peristome varies from absent to rudimentary or weakly developed.

Luisierella has been placed in the subfamily Pottioideae tribe Hyophileae by Zander (1993) because its leaf cells are bulging mammillose on the ventral surface, but plane and smooth on the dorsal surface. The genus is very similar to Weisiopsis which differs mainly in having the inflated, thin-walled basal leaf cells extend higher near the costa than at the margins. Hyophila and Plaubelia are also similar to Luisierella in having the same leaf cell morphology; they differ from it in having mostly firm-walled basal leaf cells and a stem central strand.